| Products |
| NAD and NADP Analogs |
Specialty Assays manufactures and sells a variety of NAD and NADP analogs. These
analogs in both their oxidized and reduced states exhibit several chemical and physical
properties which makes them advantageous to use in certain situations compared
with NAD and NADP. In their oxidized state they have higher oxidation potentials than
NAD and NADP, and thus enzymatic oxidations that proceed with difficulty with NAD
and NADP proceed more easily with the corresponding analogs. In their reduced
states the analogs are considerably more stable at acid, neutral, and slightly alkaline
pH values, which results in significantly greater aqueous stabilities than with NADH
and NADPH. The reduced forms also have significant absorbances in the visible region
of the spectrum and much higher molar absorptivities than NADH and NADPH.
analogs in both their oxidized and reduced states exhibit several chemical and physical
properties which makes them advantageous to use in certain situations compared
with NAD and NADP. In their oxidized state they have higher oxidation potentials than
NAD and NADP, and thus enzymatic oxidations that proceed with difficulty with NAD
and NADP proceed more easily with the corresponding analogs. In their reduced
states the analogs are considerably more stable at acid, neutral, and slightly alkaline
pH values, which results in significantly greater aqueous stabilities than with NADH
and NADPH. The reduced forms also have significant absorbances in the visible region
of the spectrum and much higher molar absorptivities than NADH and NADPH.
| Metal Chelators |
Specialty Assays has recently synthesized and developed assays for Calcium,
Magnesium, and Sodium using Phosphonazo III. Phosphonazo III is the phosphorous
analog of Arsenazo III, where the two Arsenic molecules have been replaced with two
phosphorous molecules.
Magnesium, and Sodium using Phosphonazo III. Phosphonazo III is the phosphorous
analog of Arsenazo III, where the two Arsenic molecules have been replaced with two
phosphorous molecules.
For calcium measurements, phosphonazo III offers several advantages over current
methods, which use arsenazo III and ortho-cresolphthalein complexone (CPC).
Compared with arsenazo III, phosphonazo III is about 38% more sensitive and poses
no environmental waste concerns since it contains no arsenic. Compared with CPC
methods phosphonazo III is about 300% more sensitive and unlike CPC methods,
which require a pH of ~11.7 to bind calcium, phosphonazo III binds best at a slightly
acidic pH, ~5.5. Therefore phosphonazo III reagent stability is not affected by ambient
carbon dioxide, which over time lowers the pH of CPC methods, making the reagents
unusable for calcium determinations. Also, the phosphonazo III-calcium complex has
two absorbance maxima in the visible region of the spectrum at 600 and 650 nm,
compared with CPC methods where the CPC-Ca complex absorbs around 578 nm. The
longer wavelengths of phosphonazo III are much less prone to sample spectral
interferences, especially those found in biological specimens.
methods, which use arsenazo III and ortho-cresolphthalein complexone (CPC).
Compared with arsenazo III, phosphonazo III is about 38% more sensitive and poses
no environmental waste concerns since it contains no arsenic. Compared with CPC
methods phosphonazo III is about 300% more sensitive and unlike CPC methods,
which require a pH of ~11.7 to bind calcium, phosphonazo III binds best at a slightly
acidic pH, ~5.5. Therefore phosphonazo III reagent stability is not affected by ambient
carbon dioxide, which over time lowers the pH of CPC methods, making the reagents
unusable for calcium determinations. Also, the phosphonazo III-calcium complex has
two absorbance maxima in the visible region of the spectrum at 600 and 650 nm,
compared with CPC methods where the CPC-Ca complex absorbs around 578 nm. The
longer wavelengths of phosphonazo III are much less prone to sample spectral
interferences, especially those found in biological specimens.
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| Services |
Custom Metal Chelator Synthesis - We specialize in the synthesis of Bis(Arylazo)
Derivatives of Chromotropic Acid.
Derivatives of Chromotropic Acid.
Custom Diagnostic Test Kit Development - Specialty Assays has considerable
experience and expertise in development of stable liquid diagnostic reagents using
NAD(P) and NAD(P)H analogs.
experience and expertise in development of stable liquid diagnostic reagents using
NAD(P) and NAD(P)H analogs.
| Products and Services |
| Substrates |
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| Product Disclaimer: All Specialty Assays Inc. products are intended for in vitro diagnostic or research use only. Our products are not for human consumption. |